Primer sequences used to amplify regions of the bovine FSHR gene that contain DNA polymorphisms and restriction enzymes (RE) used for genotyping. Identification of DNA polymorphisms in the bovine FSHR gene.Īge238-TableS3.pdfapplication/PDF, 37.8 KB Primer sequences and PCR conditions used to amplify each bovine FSHR exons.Īge238-TableS2.pdfapplication/PDF, 10.1 KB Representative genotyping results for the three non-synonymous polymorphisms observed in the bovine FSHR gene.Īge238-TableS1.pdfapplication/PDF, 35.5 KB FilenameĪge238-FigureS1.pdfapplication/PDF, 93.5 KBįigure S1. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Our results suggest that specific alleles of the bovine FSHR gene are associated with variations in embryo yield and in the number of unfertilised oocytes.Īs a service to our authors and readers, this journal provides supporting information supplied by the authors. SNP c.1973C>G corresponds to a threonine-to-serine (p.The658Ser) modification in the intracellular carboxyl-terminal domain of the FSHR protein, and homozygous GG Holstein cows were associated with a lower embryo yield and a higher percentage of unfertilised oocytes.
SNP c.871A>G results in an isoleucine-to-valine (p.Ile291Val) modification, and homozygous AA animals present lower embryo yield after superovulatory treatments. PCR-RFLP analyses showed that homozygous GG Holstein cows present a higher percentage of viable embryos, whereas GG and CG animals have less unfertilised oocytes. SNP c.337C>G encodes for a proline-to-alanine (p.Pro113Ala) amino acid replacement in the extracellular ligand-binding domain of the receptor. Three were non-synonymous mutations (c.337C>G, c.871A>G and c.1973C>G). Sequencing of FSHR exons 1–10 revealed seven SNPs. Our objectives were to identify SNPs in the coding region of the bovine FSHR gene and to investigate the effect of FSHR genotypes on superovulatory response in Holstein cattle. In women, genotyping for the p.N680S single nucleotide polymorphism (SNP) in the follicle-stimulating hormone receptor ( FSHR) gene may help identify poor responders before ovarian stimulation is initiated. We found your location using your IP address or because you entered it during a previous visit to Apple.In dairy cows, there is evidence that failure to respond to superovulation protocols is a heritable trait. †† We use your location to show you delivery options faster. HomePod requires some time to calibrate the sensors immediately after starting up before results are displayed. Temperature and humidity sensing is optimised for indoor domestic settings, when ambient temperatures are around 15º C to 30º C and relative humidity is around 30% to 70%. Accuracy may decrease in some situations where audio is playing for an extended period of time at high volume levels.It requires all Apple devices that access the home to be using the latest software.
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